The expression of ASS1 is increased in some types of tumors, such as colorectal, ovarian, stomach, and lung cancers, while in most types of cancers, such as melanoma, prostate cancer, breast cancer, bladder cancer, hepatocellular carcinoma, mesothelioma, pancreatic cancer, nasopharyngeal carcinoma, osteosarcomas, and myxofibrosarcomas, the expression of ASS1 is decreased3–6. Here, ASS1 is linked to osteosarcoma.