The transfection of T cells, isolated either from PBLs or tumor-infiltrating lymphocytes (TILs), with caTLR4 mRNA, caCD40 mRNA, or both, enhanced the production of IFNγ and TNFα, upregulated 4-1BB and CD25, and increased the cytolytic activity against autologous melanoma cells in vitro [202, 203]. The gene discussed is IFNG; the disease is melanoma.