We further investigated whether SIRT3 regulates the MMP during infection, and found that stable overexpression of SIRT3-GFP results in increased JC-1 fluorescence ratio early in infection (6 and 24 hpi) when compared to control cells expressing GFP fused to a mitochondrial localization signal (Mito-GFP) (Fig 2F). Here, SIRT3 is linked to infection.