We used two pancreatic cancer cell lines, SW1990 and CFPAC1, which relatively express lower levels of NUCB1 compared to HPDE, a normal pancreatic duct epithelial cell line (Supplementary Figure 2A), to generate stable cells with overexpression of NUCB1 or control vector (Supplementary Figures 2A,B). Here, NUCB1 is linked to familial pancreatic carcinoma.