To determine whether the additive effects of NUCB1 with GEM in pancreatic cancer cells were linked to regulation of the UPR and autophagy, SW1990 cells overexpressing NUCB1 or control vector were treated with GEM (Figure 4A), and changes in UPR- and autophagy-associated genes were examined by Western blot. This evidence concerns the gene NUCB1 and familial pancreatic carcinoma.