Therefore, flagellin can also be used as an immune adjuvant, mainly based on the ability to activate DCs and non‐intrinsic immune cells (such as epithelial cells and lymphatic stromal cells).[52] The interaction of flagellin and TLR5 results in the expression of varied proinflammatory cytokines, NO, H2O2, chemokines, and host defense proteins.[53] It is revealed that engineered Salmonella in the TME exerts a strong anti‐tumor efficacy through the activation of the TLR4 and TLR5 pathways. This evidence concerns the gene TLR4 and neoplasm.