EBF1 and cholangiocarcinoma: Among 56 EBF1 probes, 11 of 11 probes located at EBF1 promoter region (TSS1500 and first exon) were significantly hypermethylated in CCA tumors compared with normal bile ducts (Figure 1B), and the methylation levels of 4 (located at TSS1500) of 11 probes were negatively correlated with the EBF1 mRNA expression levels as shown in Table S4.