EBF1 and cholangiocarcinoma: After 5-Aza-dC-treatment, CCA cell lines (KKU-213A and KKU-213B) showed significant reduction of the DNA methylation rates as shown in Figure 3A and Figure 3B. Notably, significant increases of EBF1 mRNA expression were observed in 5-Aza-dC treated-KKU-213A and KKU-213B cells (Figure 3C).