The occurrence of HCC is associated with the activation of many signaling pathways, such as AKT signaling pathway, Wnt signaling pathway, and PI3K/AKT signaling pathway, etc. We found that compared with CK19-HCC, PI3K-Akt signal pathway (hsa04151) is more likely to be altered in DPHCC (80% for DPHCC, 21.4% for CK19-HCC, P = 0.011) (Figure 3B). Here, AKT1 is linked to hepatocellular carcinoma.