As results, LAG-3, CTLA-4, PD-1, and PD-L1 were enriched in in HCC samples with CENPFhigh, compared with CENPFlow or normal groups, but no significant positive correlation was found between expression of LAG3, CTLA4, HAVCR2, PD-1, and PD-L1 and the expression of CENPF. This evidence concerns the gene CTLA4 and hepatocellular carcinoma.