Among the four hematological malignant cell types, T-cell acute lymphoblastic leukemia (T-ALL) cell line Molt4 was the most susceptible to TfR-CAR T cell-mediated cytolysis, followed by chronic myeloid leukemia cell line K562, myeloma cell line U266 and acute myeloid leukemia cell line Kg1a, although TfR expression level was comparable among these cell types. The gene discussed is TFRC; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.