Interestingly, while the ratio of acetylated to total tau in AD autophagic vacuoles was comparable between control and AD, in support of higher overall entrapment of tau by autophagosomes, we noticed a marked shift to an increase in the fraction of acetylated tau in the LE/MVB isolated from AD brains (Fig. 5g, i and Supplemental Fig. 5d). Here, MAPT is linked to Alzheimer disease.