In addition, there was a significant elevation of THAP9-AS1 expression in ESCC cell lines (KYSE-150, YES2, TE-1, Eca-109, KYSE-30) as compared to that in normal human esophageal epithelial cell line Het-1A, especially in Eca-109 and KYSE-30 (Fig. 1F). This evidence concerns the gene THAP9 and esophageal squamous cell carcinoma.