TWIST1 and cancer: While the early KPC;ST PDAC cells were comparable to the early KPC PDAC cells in showing predominantly epithelial cluster phenotypes (clusters 1–26), the late-stage KPC;ST PDAC cells significantly maintained/stabilized epithelial cluster phenotypes (clusters 1–26) due to the loss of EMT-promoting Snail and Twist in the cancer cells (Figures 2E and S2B–S2F).