As described above, NR5A2 silencing inhibited pancreatic cancer cell proliferation, migration, and invasion, we sought to determine whether NR5A2 overexpression would increase tumor cell malignancy by stably overexpressing NR5A2 in AsPC-1 and HPAC cells (Fig. 3A, B). Here, NR5A2 is linked to familial pancreatic carcinoma.