CGAS and infection: Quantification of JCV VP1-positive cells showed that JCV replication was greatly enhanced in RIG-I-KO and cGAS-KO SVGA cells compared to that of NT control cells, where cGAS predominantly contributed to JCV restriction early during infection (at 6 days postinfection [dpi]), while both cGAS and RIG-I equally contributed to JCV restriction later during infection (at 12 dpi) (Fig. 1D and E).