In T3M4 cells—which exhibit baseline IRF3(S396) phosphorylation and ISG expression in the absence of PVSRIPO infection/poly(I:C) transfection—the response to YTHDF3 depletion was broadly reduced p-STAT1(Y701) and ISG expression at baseline (0 hpi) and at 8 hpi (Fig. 3E). This evidence concerns the gene IRF3 and infection.