Similar to that in primary human LUAD tissues, both PD-L1-lnc and PD-L1 mRNA were detected in various human lung cancer cells by agarose gel electrophoresis using PD-L1 mRNA/PD-L1-lnc primer 2 (Fig. 2c) or by RNA BaseScope (Fig. 2d) and RT-PCR using PD-L1 mRNA-specific primer 3 or PD-L1-lnc-specific primer 4 (Fig. 2e). This evidence concerns the gene CD274 and lung carcinoma.