Damaged organelles and misfolded proteins are cleared by (macro)autophagy, which is modulated by H2S. Exogenous H2S (NaHS) inhibits autophagy in neonatal rat cardiomyocytes in an in vitro hypoxia-reoxygenation (HR) model, via PI3K/GSK3β signaling [84] and also in hepatocellular carcinoma (HCC) cells, as illustrated by downregulation of genes in the PI3K/AKT/mTOR pathway[85]. This evidence concerns the gene GSK3B and hepatocellular carcinoma.