Here, viral DNA was detected in cervical cancer patients using the universal primers GP5+/6+, and the PRDM4 IRS was 3.86 ± 0.82 in HPV-positive and 4.13 ± 1.31 in HPV-negative samples, indicating that there was no significant difference between PRDM4 expression and HPV infection (Supplementary Table 1). This evidence concerns the gene PRDM4 and cervical cancer.