CD14 and infection: Despite using our optimised ex vivo derived skin MNP culture methods39, we were unable to measure direct infection of CD14-expressing cell subsets by flow cytometry (as we have done previously with other ex vivo derived skin MNPs13,21) as only small cell yields could be derived for each specific cell subsets (particularly ex vivo MDDCs), even from very large pieces of abdominal skin, and too few live cells could be detected after 96 h of culture.