To characterize infiltrated immune cells, especially B cells in human breast cancer, we purified hCD45+ cells from surgically isolated breast cancer tissues and corresponding peripheral blood mononuclear cell (PBMC) samples from six TNBC (TNBC1–6 and PBMC1–6), three luminal A breast cancer (LABC7–9 and PBMC7–9), and one HER2-positive breast cancer (HER2BC10 and PBMC10) treatment-naive patients (Fig. 1a). This evidence concerns the gene ERBB2 and breast cancer.