Western blotting confirmed that the miR-185-3p inhibitor significantly upregulated NRP1 expression in CRC cells (Fig. 6D), and NRP1 protein levels were reduced after transfection with the miR-185-3p mimic in SW1116-shAGO2#1 and RKO-shAGO2#1 cell (Fig. 6E). Here, NRP1 is linked to colorectal carcinoma.