We found that the percentages of T cells (CD45+CD3+), B cells (CD45+CD19+), neutrophils (CD45hiCD11b+Ly6G+), and macrophages (CD45hiCD11b+Ly6G-) among singlets increased in the ipsilateral hemisphere with ischemic stroke of HSD mice (Fig. 2a), while the composition of neutrophils (CD45hiCD11b+Ly6G+) and monocytes/macrophages (CD45hiCD11b+Ly6G-) in the peripheral blood and spleen remained comparable (Supplementary Figure 1C). The gene discussed is CD19; the disease is ischemic stroke.