ACTA2 and neoplasm: Then, in order to evaluate the effects of ACTA2-AS1 in vivo experiments, the BALB/c nude mice were employed to construct tumor subcutaneous xenografts, the results suggested that the volume and weight of the xenograft tumors were larger or smaller in the sh-ACTA2-AS1 group or in OE ACTA2-AS1 group comparing the sh-NC and vector group respectively (Fig. 6a and b).