Finally, we matched the DIA against a larger multi-HLA library, called BigLib, comprising 136,458 peptide sequences identified in 190 publicly available measurements of eluted HLA-binding peptides in 40 samples from multiple and diverse biological sources, including B cells (not including JY cells), T cells, colon cancer organoids, meningioma tissues, and melanoma cell lines treated or not with interferon gamma or decitabine, covering 23 HLA-A, 27 HLA-B, and 21 HLA-C alleles. The gene discussed is HLA-C; the disease is malignant colon neoplasm.