Indeed, Daniele et al., showed that the improvement in insulin sensitivity was closely correlated with the decrease of plasmatic FA, the increases of mitochondrial ATP synthesis > 50% and of insulin-mediated glucose disposal in obese normal glucose tolerant and T2D subjects [50]. Moreover, Toledo et al. showed that skeletal muscle mitochondria are significantly resilient to nutrient overload. This evidence concerns the gene INS and type 2 diabetes mellitus.