We used CRISPR/Cas9 genome editing technology and three genetic backgrounds: Sprague Dawley (SD), Fischer 344 (F344), and Lewis (LEW) to generate new rat strains/stocks with genetic alterations in Nod2, Atg16l1 and Il23r. These models represent important new animal resources for studying the biological functions of the genes as well as their role as susceptibility loci in the context of IBD. This evidence concerns the gene NOD2 and inflammatory bowel disease.