Specifically, GINS4 expression was significantly greater in HCC patients that belong to age ≤60 years old (P = 0.011), female (P = 0.010), AFP level >400 (P = 0.007), with residual tumor (P = 0.009), relapse (P = 0.017) (Figures 5A–E). This evidence concerns the gene AFP and hepatocellular carcinoma.