PIK3CA and breast carcinoma: In the study performing 46 formalin-fixed and paraffin-embedded breast carcinoma specimens, Lambert et al. (54) compared three different RT-PCR approaches, namely the cobas® PIK3CA Mutation Test (Roche Diagnostics, Meylan, France), the PCR amplification-refractory mutation system (ARMS) Scorpion, and the High-Resolution Melting (HRM) PCR assay.