To further verify the expression pattern of the four selected candidate membrane protein targets, Western blot and Immunofluoresence (IF) analysis of HER2, STEAP4, EPHA2 and CD97 were performed from the whole cell lysates of the HCC-1954, the MDA-MB-231 and the MCF-10A cell lines. This evidence concerns the gene ERBB2 and hepatocellular carcinoma.