Since the isolated compounds showed more cytotoxic effects in human colon cancer HCT116 and human cervical HeLa cell lines in comparison to other cell lines, in-vitro HDAC inhibitory activity of the compounds 1-5 were also evaluated on these two cell lines in comparison with vorinostat as the reference drug (Table 1). The gene discussed is HDAC9; the disease is malignant colon neoplasm.