CD8A and cholangiocarcinoma: In our analysis, we used bulk sequencing data of cholangiocarcinoma patients in TCGA database to calculate the immune infiltration scores of different immune cell populations, and then we compared expression difference between groups, locating immune infiltration highly associated genes; we found PNOC was mainly expressed by infiltrated B cells, which was survival related, while LAIR2 was mainly expressed by Tregs and partial CD8+/GZMB+ T cells, indicating exhaustive immune status of T cells.