In line with these observations, we also found highly expressed Skp2 in Vem-resistant melanoma cells (Figures 1B, 2D), and decreased AKT expression and AKT phosphorylation in two single clones of VR1-SgSkp2 cells (VR1-SgSkp2-No.1 and No.2), which might be the reason to increased cell apoptosis and cell growth arrest caused by the combination regimen (Figures 2, 3C). This evidence concerns the gene AKT1 and melanoma.