The 58 liver cancer patients were divided into three groups based on the results of western analysis: 1) higher ASS1 expression in tumor vs. peritumoral tissue (n = 7); 2) lower ASS1 expression in tumor vs. peritumoral tissue (n = 51); and 3) no significant difference in ASS1 expression between tumor and nontumor tissue (n = 0). This evidence concerns the gene ASS1 and neoplasm.