Mutations of TP53, APC, KRAS, BRAF and ATM were a diagnosis signature for CRC; TP53 and BRAF, a mutation signature for the pathological classification; TP53, PIK3CA, FAT4, FMN2 and TRRAP, a mutation signature for tumor stage; PIK3CA, LRP1B, FAT4 and ROS1, a mutation signature for prognosis. The gene discussed is ROS1; the disease is colorectal carcinoma.