In addition, we used logistic regression analysis in validation cases to get the coefficient of the pathological classification = 3.192 × (mutation of TP53) - 2.954 × (mutation of BRAF) + 1.493, and mutation of TP53 or BRAF = 0 (mutated) or 1 (no mutated); and mucinous adenocarcinoma (the coefficient = 0) and other adenocarcinoma (the coefficient = 1). The gene discussed is TP53; the disease is adenocarcinoma.