To mimic the process of infected activated CD4+ T cells transitioning back to a more resting state, and monitor how the dynamics of productive and latent infection change during this process, DFV-B-infected cells were transferred to cytokine-free medium (CFM) on day 3 p.i. As culture time increased, the proportion of DP cells decreased significantly, while the proportion of mC cells gradually increased (Figure 1C). Here, CD4 is linked to disease arising from reactivation of latent virus.