These results indicate that treatment with TMS enhanced intracellular ROS generation, induced DNA damages, and activated the p53/PUMA/Bax signal pathway, resulting in the enhanced outer membrane of mitochondria permeabilization and caspase activation, as well as sensitizing osteosarcoma cell to TRAIL-mediated apoptotic cell death (Figure 10). This evidence concerns the gene BBC3 and osteosarcoma.