As an anticancer agent, FCFNCs displayed the highest apoptotic impact (> 77.7%) on Caco-2, HepG-2, MCF-7 and PC-3 cancer cells at IC50 ≤ 17.21 μg/mL with the maximum up regulation of p53 and caspase 3 expression and the lowest Ki-67 level, relative to both functionalized CuNPs (FCNPs) and FeNPs (FFNPs). The gene discussed is TP53; the disease is cancer.