CREB1 and Anxiety: Here, we found that juvenile FLX pretreatment resulted in a long-term decrease in the phosphorylation of ERK2 and CREB within both brain regions (Fig. 5A, 6A, respectively), with additional decreases in total BDNF protein (both proBDNF and mBDNF) within the prefrontal cortex (Fig. 6A); molecular changes that likely underlie the female-specific anxiety-like behavioral phenotype observed in adulthood.