We studied the effects of IT protocol on RBP4, aHSG/fetuin-A, FGF21, and CRP levels, liver histology, HOMA-IR as well as total lipid content in the obese Zucker (Crl:ZUC(ORL)-Leprfa) rats—an animal model of insulin resistance, glucose intolerance, metabolic syndrome, and genetic obesity27. This evidence concerns the gene FGF21 and Insulin resistance.