OCLN and infection: The group supplemented with 1% thymol nanoemulsion showed the most significant (p < 0.05) upregulation of occludin with 2.93 and 2.70-fold pre- and post-infection (Fig. 2a,b) and JAM with 2.77 and 2.26-fold pre- and post-infection c,d, respectively when compared with control groups.