CSF2 and colorectal carcinoma: ELISA assays further verified the significant increase of CSF2 in CM derived from KRAS mutant CRC cells (SW620, HCT116, SW480, SW48-KRASG12V) compared to CM from KRAS wild-type cells (Colo320, Caco2, SW48, SW620-siKRAS) (Fig. 4b–d), whereas a similar pattern was not observed for G-CSF and IL-7 (Supplementary Fig. 5c).