DDIT4 and ischemic stroke: Representative images showing the immunofluorescence staining of DDIT4 and Beclin-1 after the groups had been transfected with pcDNA-C2dat2, pcDNA-NC, si-C2dat2, or si-NT followed by OGD/R for 24 h also conformed with the above results, suggesting that C2dat2 knockdown strikingly suppressed autophagy in ischemic stroke (Figure 4C–4E).