PGRMC1 and triple-negative breast carcinoma: To investigate whether the breast-specific regulation of Pgrmc1 affects the migratory activity of triple-negative breast cancer cells, we used highly invasive MDA-MB-231 cells and found that transfection with PGRMC1 siRNA also resulted in decreased cell migration in MDA-MB-231 cells according to cell scratch assay (p < 0.05, 74.1% compared with Control siRNA group) (Fig. 6a) and transwell assay (p < 0.05, 51% compared with Control siRNA) (Fig. 6b).