Lastly, the present study is in vitro and although there have been some in vivo studies examining the role of IL-2 in Treg-mediated neuroprotection in the gray matter (Hu et al., 2014), the efficacy of the IL-2/IL-2R-treated Tregs in mitigating white matter injury specifically is yet to be explored in stroke animal models. This evidence concerns the gene IL2RA and stroke disorder.