Overweight individuals (BMI ≥ 25) have higher Brd4 expression in blood samples than individuals with normal weight, and the expression levels of Brd4 positively correlate with the expression of Gdf3 in obese individuals (Figure 5, H and I), highlighting the importance of Brd4-Gdf3 regulatory pathway in obesity. This evidence concerns the gene GDF3 and obesity due to melanocortin 4 receptor deficiency.