In total, 15 distinct cell clusters were identified by clustering analysis and classified based on the top 10 DEGs, which included CD4+T cells, CD8+ T cells, cancer stem cells, plasma cells, natural killer cells, M1 macrophages, macrophages, M2 macrophages, regulatory B cells, T helper 17 (Th17) cells, dendritic cells, effector T cells, cancer cells, endothelial cells, and Th2 cells (Figure 1E). This evidence concerns the gene CD8A and cancer.