Immunoblot analysis revealed that the conversion of LC3-I to LC3-II was considerably elevated, along with the expression levels of Beclin-1 and ATG7; moreover, the expression of p62 was markedly inhibited in si-NDUFA4L2-transfected LN229 and GBM-XX cells (Fig. 2G). This evidence concerns the gene BECN1 and glioblastoma.