The following detection of cell phenotypes indicated not only that MCM8 overexpression promotes cell proliferation (Fig. 6A), colony formation (Fig. 6B), and cell migration (Fig. 6C, D) as well as inhibits cell apoptosis (Fig. 6E) but also that CTGF knockdown could partially reverse the MCM8-induced regulatory effects on osteosarcoma cells (Fig. 6). This evidence concerns the gene CCN2 and osteosarcoma.