To examine if the identified deletion mutation (c.1725_1733del) was associated with the tumor response to toceranib, the phosphorylation status of the wild-type and mutant KIT and the effects of toceranib on the phosphorylation of mutant KIT were analyzed using the human embryonic kidney (HEK) 293 cell line (provided by Dr. Tanaka, Nippon Veterinary and Life Science University), as previously described [15]. This evidence concerns the gene KIT and neoplasm.