This was manifested by an increase in CD8+ TIL cells, Neutrophil (Ly6G + cells), tumor-specific T cells, Dendritic cells (CD11c+), high-mobility group box 1 (HMGB1), and activated CD8+ T cells (as indicated by intracellular interferon gamma (IFNγ) expression)) at 7–10 days post-treatment (Figure 6(H)). Here, HMGB1 is linked to neoplasm.